WhiteRotV1, Main, Exploration, bibRecord, 000396

Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor.

Identifieur interne : 000396 ( Main/Exploration ); précédent : 000395; suivant : 000397

Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor.

Auteurs : Mathieu Schwartz [France] ; Thomas Perrot [France] ; Emmanuel Aubert [France] ; Stéphane Dumarçay [France] ; Frédérique Favier [France] ; Philippe Gérardin [France] ; Mélanie Morel-Rouhier [France] ; Guillermo Mulliert [France] ; Fanny Saiag [France] ; Claude Didierjean [France] ; Eric Gelhaye [France]

Source :

Scientific reports [ 2045-2322 ] ; 2018.

RBID : pubmed:29855494

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Benzophenones (chemistry), Benzophenones (metabolism), Binding Sites (MeSH), Crystallography, X-Ray (MeSH), Flavonoids (chemistry), Flavonoids (metabolism), Fungal Proteins (chemistry), Fungal Proteins (metabolism), Glutathione Transferase (chemistry), Glutathione Transferase (metabolism), Isoenzymes (chemistry), Isoenzymes (metabolism), Kinetics (MeSH), Metabolic Detoxication, Phase II (MeSH), Polyphenols (chemistry), Polyphenols (metabolism), Protein Stability (MeSH), Protein Structure, Tertiary (MeSH), Prunus (chemistry), Prunus (metabolism), Sequence Alignment (MeSH), Temperature (MeSH), Trametes (metabolism), Wood (chemistry), Wood (metabolism).
MESH :
- chemical , chemistry : Benzophenones, Flavonoids, Fungal Proteins, Glutathione Transferase, Isoenzymes, Polyphenols.
- chemical , metabolism : Benzophenones, Flavonoids, Fungal Proteins, Glutathione Transferase, Isoenzymes, Polyphenols.
- chemistry : Prunus, Wood.
- metabolism : Prunus, Trametes, Wood.
- Amino Acid Sequence, Binding Sites, Crystallography, X-Ray, Kinetics, Metabolic Detoxication, Phase II, Protein Stability, Protein Structure, Tertiary, Sequence Alignment, Temperature.

Abstract

Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.

DOI: 10.1038/s41598-018-26601-3
PubMed: 29855494
PubMed Central: PMC5981210

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000406
to stream Main, to step Curation: 000406

Le document en format XML

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<author><name sortKey="Morel Rouhier, Melanie" sort="Morel Rouhier, Melanie" uniqKey="Morel Rouhier M" first="Mélanie" last="Morel-Rouhier">Mélanie Morel-Rouhier</name>
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<author><name sortKey="Mulliert, Guillermo" sort="Mulliert, Guillermo" uniqKey="Mulliert G" first="Guillermo" last="Mulliert">Guillermo Mulliert</name>
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<country xml:lang="fr">France</country>
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<author><name sortKey="Saiag, Fanny" sort="Saiag, Fanny" uniqKey="Saiag F" first="Fanny" last="Saiag">Fanny Saiag</name>
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<author><name sortKey="Didierjean, Claude" sort="Didierjean, Claude" uniqKey="Didierjean C" first="Claude" last="Didierjean">Claude Didierjean</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Benzophenones (chemistry)</term>
<term>Benzophenones (metabolism)</term>
<term>Binding Sites (MeSH)</term>
<term>Crystallography, X-Ray (MeSH)</term>
<term>Flavonoids (chemistry)</term>
<term>Flavonoids (metabolism)</term>
<term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Glutathione Transferase (chemistry)</term>
<term>Glutathione Transferase (metabolism)</term>
<term>Isoenzymes (chemistry)</term>
<term>Isoenzymes (metabolism)</term>
<term>Kinetics (MeSH)</term>
<term>Metabolic Detoxication, Phase II (MeSH)</term>
<term>Polyphenols (chemistry)</term>
<term>Polyphenols (metabolism)</term>
<term>Protein Stability (MeSH)</term>
<term>Protein Structure, Tertiary (MeSH)</term>
<term>Prunus (chemistry)</term>
<term>Prunus (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Temperature (MeSH)</term>
<term>Trametes (metabolism)</term>
<term>Wood (chemistry)</term>
<term>Wood (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term>
<term>Benzophénones (composition chimique)</term>
<term>Benzophénones (métabolisme)</term>
<term>Bois (composition chimique)</term>
<term>Bois (métabolisme)</term>
<term>Cinétique (MeSH)</term>
<term>Cristallographie aux rayons X (MeSH)</term>
<term>Détoxication de phase II (MeSH)</term>
<term>Flavonoïdes (composition chimique)</term>
<term>Flavonoïdes (métabolisme)</term>
<term>Glutathione transferase (composition chimique)</term>
<term>Glutathione transferase (métabolisme)</term>
<term>Isoenzymes (composition chimique)</term>
<term>Isoenzymes (métabolisme)</term>
<term>Polyphénols (composition chimique)</term>
<term>Polyphénols (métabolisme)</term>
<term>Protéines fongiques (composition chimique)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Prunus (composition chimique)</term>
<term>Prunus (métabolisme)</term>
<term>Sites de fixation (MeSH)</term>
<term>Stabilité protéique (MeSH)</term>
<term>Structure tertiaire des protéines (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Température (MeSH)</term>
<term>Trametes (métabolisme)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzophenones</term>
<term>Flavonoids</term>
<term>Fungal Proteins</term>
<term>Glutathione Transferase</term>
<term>Isoenzymes</term>
<term>Polyphenols</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzophenones</term>
<term>Flavonoids</term>
<term>Fungal Proteins</term>
<term>Glutathione Transferase</term>
<term>Isoenzymes</term>
<term>Polyphenols</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Prunus</term>
<term>Wood</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Benzophénones</term>
<term>Bois</term>
<term>Flavonoïdes</term>
<term>Glutathione transferase</term>
<term>Isoenzymes</term>
<term>Polyphénols</term>
<term>Protéines fongiques</term>
<term>Prunus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Prunus</term>
<term>Trametes</term>
<term>Wood</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Benzophénones</term>
<term>Bois</term>
<term>Flavonoïdes</term>
<term>Glutathione transferase</term>
<term>Isoenzymes</term>
<term>Polyphénols</term>
<term>Protéines fongiques</term>
<term>Prunus</term>
<term>Trametes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Binding Sites</term>
<term>Crystallography, X-Ray</term>
<term>Kinetics</term>
<term>Metabolic Detoxication, Phase II</term>
<term>Protein Stability</term>
<term>Protein Structure, Tertiary</term>
<term>Sequence Alignment</term>
<term>Temperature</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Cinétique</term>
<term>Cristallographie aux rayons X</term>
<term>Détoxication de phase II</term>
<term>Sites de fixation</term>
<term>Stabilité protéique</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Température</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en">Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.</div>
</front>
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<DateCompleted><Year>2019</Year>
<Month>10</Month>
<Day>08</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>10</Month>
<Day>08</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>8</Volume>
<Issue>1</Issue>
<PubDate><Year>2018</Year>
<Month>05</Month>
<Day>31</Day>
</PubDate>
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<Title>Scientific reports</Title>
<ISOAbbreviation>Sci Rep</ISOAbbreviation>
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<ArticleTitle>Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor.</ArticleTitle>
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<Abstract><AbstractText>Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schwartz</LastName>
<ForeName>Mathieu</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, CNRS, CRM2, Nancy, France.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Perrot</LastName>
<ForeName>Thomas</ForeName>
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<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, Nancy, France.</Affiliation>
</AffiliationInfo>
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<AffiliationInfo><Affiliation>Université de Lorraine, LERMAB, Nancy, France.</Affiliation>
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<ForeName>Frédérique</ForeName>
<Initials>F</Initials>
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</AffiliationInfo>
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<ForeName>Mélanie</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, Nancy, France.</Affiliation>
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<Initials>G</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, CNRS, CRM2, Nancy, France.</Affiliation>
</AffiliationInfo>
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<ForeName>Fanny</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Didierjean</LastName>
<ForeName>Claude</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, CNRS, CRM2, Nancy, France. claude.didierjean@univ-lorraine.fr.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Gelhaye</LastName>
<ForeName>Eric</ForeName>
<Initials>E</Initials>
<Identifier Source="ORCID">0000-0002-0699-9113</Identifier>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, Nancy, France. eric.gelhaye@univ-lorraine.fr.</Affiliation>
</AffiliationInfo>
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<Language>eng</Language>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>05</Month>
<Day>31</Day>
</ArticleDate>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
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<MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D050217" MajorTopicYN="Y">Metabolic Detoxication, Phase II</DescriptorName>
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<MeshHeading><DescriptorName UI="D059808" MajorTopicYN="N">Polyphenols</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D055550" MajorTopicYN="N">Protein Stability</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D027861" MajorTopicYN="N">Prunus</DescriptorName>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
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<MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName>
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<MeshHeading><DescriptorName UI="D055454" MajorTopicYN="N">Trametes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName>
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<ArticleIdList><ArticleId IdType="pubmed">29855494</ArticleId>
<ArticleId IdType="doi">10.1038/s41598-018-26601-3</ArticleId>
<ArticleId IdType="pii">10.1038/s41598-018-26601-3</ArticleId>
<ArticleId IdType="pmc">PMC5981210</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2008 Aug;D64(Pt 8):883-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18645237</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2011 Aug 5;333(6043):762-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21764756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Apr 09;8(4):e60324</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23593192</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Mol Life Sci. 2009 Dec;66(23):3711-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19662500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2013 Sep 6;438(4):588-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23939046</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Drug Metab Rev. 2011 May;43(2):138-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21428697</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2000 Aug 11;275(32):24798-806</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10783391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Environ Manage. 2012 Aug 15;104:114-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22487399</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Open Bio. 2016 Dec 22;7(2):122-132</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28174680</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytochem Anal. 2003 Nov-Dec;14(6):371-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14667064</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochimie. 2013 Feb;95(2):336-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23063695</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Insect Mol Biol. 2005 Jan;14(1):3-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15663770</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 2004 Sep 1;332(1):153-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15301960</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2015 Sep;20(9):576-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26205169</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W615-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15215462</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Insect Sci. 2016 Feb;13:70-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27436555</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21460441</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2256-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15572779</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2014 Apr 22;12(4):e1001843</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24756107</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1983 Aug 12;221(4611):661-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17787736</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Chem Phys. 2010 Apr 21;132(15):154104</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20423165</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2015 Oct;83:103-112</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26348000</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2002 Jun 4;41(22):7008-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12033934</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2015 Oct 01;10(10):e0137083</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26426695</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2010 Nov 19;285(47):36322-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20841361</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microb Biotechnol. 2013 May;6(3):248-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23279857</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Prod Rep. 2014 Sep;31(9):1158-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24972079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr B. 2008 Apr;64(Pt 2):206-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18369292</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Chem Ecol. 2013 Sep;39(9):1232-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24036972</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Nat Prod. 2016 Aug 26;79(8):1962-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27498895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Jun 29;336(6089):1715-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22745431</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2015 Aug;71(Pt 8):1777-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26249358</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15572765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Jan 22;9(1):e86683</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24466198</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Nov 20;8(11):e80298</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24278272</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Enzyme Inhib Med Chem. 2017 Dec;32(1):355-362</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28097907</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2011 Mar;9(3):177-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21297669</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2017 Jan;34(1):35-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27834665</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2018 Apr 6;293(14 ):4955-4968</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29449375</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytochemistry. 2009 Dec;70(17-18):2040-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19837443</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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</settlement>
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</orgName>
</list>
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Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor.

Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor.

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